How to Reconstitute Peptides: Complete Step-by-Step Guide

Proper peptide reconstitution is essential for maintaining peptide integrity and accurate dosing. This guide walks you through every step.

What You'll Need

• Lyophilized (freeze-dried) peptide vial
• Bacteriostatic water (BAC water) — NOT sterile water for most peptides
• Insulin syringes (29-31 gauge)
• Alcohol wipes

Step-by-Step Reconstitution

Step 1: Prepare Your Workspace

Clean surface, wash hands, have all supplies ready. Wipe the tops of both the peptide vial and BAC water vial with alcohol.

Step 2: Draw Bacteriostatic Water

Using an insulin syringe, draw your desired amount of BAC water. Common amounts: 1mL or 2mL depending on your dosing preference.

Step 3: Add Water to Peptide

Critical: Aim the stream of water down the side of the vial, not directly onto the peptide powder. Let it roll gently down the glass. Never shake or agitate.

Step 4: Gentle Swirling

Roll the vial gently between your palms. The peptide should dissolve within 1-5 minutes. If cloudiness persists, refrigerate and check in 30 minutes.

Step 5: Storage

Refrigerate at 36-46°F (2-8°C). Most reconstituted peptides last 28-30 days refrigerated. Never freeze reconstituted peptides.

Dosing Math Made Simple

Peptide Amount	BAC Water Added	Concentration	250mcg = ?
5mg	1mL	5mg/mL	5 units (0.05mL)
5mg	2mL	2.5mg/mL	10 units (0.10mL)
10mg	1mL	10mg/mL	2.5 units (0.025mL)
10mg	2mL	5mg/mL	5 units (0.05mL)

Common Mistakes

• Spraying directly onto powder: This damages the peptide chains
• Shaking the vial: Use gentle rolling only
• Room temperature storage: Always refrigerate after reconstitution
• Using expired BAC water: Check expiration dates
• Freezing reconstituted peptides: Ice crystals destroy the molecular structure