Can I use sterile water instead of bacteriostatic water?

Technically yes, but it is not recommended. Sterile water contains no preservative, meaning the reconstituted peptide must be used within 24-48 hours and cannot be safely drawn from multiple times. Bacteriostatic water contains 0.9% benzyl alcohol, which prevents bacterial growth and allows multi-dose use for 4-6 weeks when refrigerated.

How do I calculate my peptide draw volume?

Formula: Draw volume (ml) = Dose (mcg) ÷ Concentration (mcg/ml). Example: You want 250mcg from a 5mg/2ml vial. Concentration = 5000mcg ÷ 2ml = 2500 mcg/ml. Draw = 250 ÷ 2500 = 0.1ml = 10 units on an insulin syringe.

How long does reconstituted peptide last?

Reconstituted with bacteriostatic water and refrigerated at 2-8°C: 4-6 weeks. With sterile water: 24-48 hours max. Signs of degradation: cloudiness, particulate matter, or color change. Discard immediately if any are observed. Never freeze reconstituted peptides.

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Reconstitution Guide

Step-by-step guide to reconstituting peptides with BAC water. Calculate exact syringe draw volumes for precise dosing.

Quick Select Peptide

Dose Calculator

Vial Size (mg)

BAC Water (mL)

Dose (mcg)

Enter vial size, BAC water volume, and desired dose to calculate.

Quick Reference — Common Reconstitutions

Peptide	Vial	BAC Water	Dose	Draw	Doses/Vial
BPC-157	5mg	2mL	250mcg	10.0u	20
TB-500	5mg	2mL	2500mcg	100.0u	2
CJC-1295 / Ipamorelin	5mg	2.5mL	300mcg	15.0u	16
Semaglutide	5mg	2mL	500mcg	20.0u	10
GHK-Cu	5mg	2mL	200mcg	8.0u	25
Tesamorelin	2mg	2mL	2000mcg	200.0u	1
PT-141	10mg	2mL	2000mcg	40.0u	5
MOTS-C	10mg	2mL	5000mcg	100.0u	2
Epitalon	10mg	2mL	5000mcg	100.0u	2

Peptide Dosing Calculator Original Peptide Calculator Peptide Guides

This peptide reconstitution calculator determines the exact volume of bacteriostatic water to add to your lyophilized peptide vial and calculates your draw volume for any target dose. Enter your vial size (mg), desired water volume, and target dose to get precise syringe markings.

Medical Disclaimer

The information and calculations provided by this tool are for educational and research purposes only. Peptides discussed on NinjAthlete are research compounds and are not FDA-approved for human consumption, treatment, cure, or prevention of any disease.

This tool does not provide medical advice, diagnosis, or treatment recommendations. Dosing outputs are based on published research protocols and general pharmacological principles — they are not a prescription or a substitute for guidance from a licensed physician, pharmacist, or qualified healthcare provider.

Always consult a licensed medical professional before using any peptide or research compound. NinjAthlete assumes no liability for decisions made based on the output of this tool. Read our editorial policy

Frequently Asked Questions

The standard is 2ml of bacteriostatic water for a 5mg vial, giving you a concentration of 2,500 mcg/ml (250mcg per 0.1ml / 10 units on an insulin syringe). You can use 1ml for double concentration or 2.5ml for easier math. The amount is flexible — it only changes how much you draw per dose.

Related Tools & Guides

Peptide Dosing Calculator How to Reconstitute Peptides Guide Why Mixing Peptides Sabotages Results Peptide Timing Guide

NinjAthlete Team

NinjAthlete is a performance optimization platform publishing evidence-based guides on HYROX training, peptide therapy, biohacking, and longevity science. HYROX content is informed by sub-60 competition experience (59:51, HYROX Dallas). Peptide content cites peer-reviewed research with DOI/PubMed links.

Conflict of interest disclosure: NinjAthlete has affiliate relationships with peptide research suppliers. Discount codes may earn NinjAthlete a commission. This does not influence dosing methodology, research citations, or educational content.

Last reviewed: April 2026|Editorial Policy

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